Coding

Part:BBa_K4695040

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-01)


docxylY

Coding sequence for dockerin domain (DocXylY) from the cellulosome of Acetovibrio thermocellus.

Design Notes

FLANKED 5' fusion site: TTCG, 3' fusion site: CGGC.
Source sequence was optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

This part has no START codon at the 5' end of the sequence and no codon STOP at the 3' end because it was designed as a part of a fusion protein with interchangable domains. If you want to express only this coding sequence, please add the START and STOP codons in your design.

Source

Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None